Human Monocytes - CD14, CD16 - Ziegler-Heitbrock


Markers and Staining Protocols Non-Human Primate Monocytes

Markers that can be used for identification of monocyte subsets in are DR,CD14, CD16 (Sugimoto, J Immunol, 195,1774, 2015) and slan (see Hofer et al, Frontiers in Immunology, Article 2052, 2019). For CD14 there are clones, which do not cross-react in all monkeys tested while other clones do not react with selected NHPs. As given under the antibody TUK4 shows reactivity with cells from Rhesus but does not react with monocytes from Marmosets. The data base can be helpful, when selecting reagents for the analysis of monocytes in a given species.

The staining protocols listed under Human Monocytes can be applied to NHP whole blood or isolated mononuclear cells. A large panel of additional cross-reactive monoclonal antibodies are available. Antibodies, which have been successfully used in the study of non-human primate blood monocytes, include CD11b and c, CD45, anti-CCR2, CD64, CD68, CD80, CD86 and CD163.