Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Single-Cell Sleuthing: Cracking the Monocyte Code for Cardiovascular Clues.

Abstract

Human monocytes were first isolated as Fc receptor-positive and Fc receptor-negative subsets by Zembala and Pryma in 1984. A second seminal discovery in human monocyte biology was the discovery of human clusters of differentiation (CD) 16+ monocytes in 1989 using 2-color flow cytometry by Passlick and Ziegler-Heitbrock. In 2010, the Nomenclature Committee of the International Union of Immunological Societies proposed the classical (CD14+), intermediate (CD14+CD16+), and nonclassical (CD16+) nomenclature for human monocytes. In 2003, Geissmann and Jung first reported on the mouse equivalent of the human monocyte subsets.