Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock


Young infants display heterogeneous serological responses and extensive but reversible transcriptional changes following initial immunizations.


Infants necessitate vaccinations to prevent life-threatening infections. Our understanding of the infant immune responses to routine vaccines remains limited. We analyzed two cohorts of 2-month-old infants before vaccination, one week, and one-month post-vaccination. We report remarkable heterogeneity but limited antibody responses to the different antigens. Whole-blood transcriptome analysis in an initial cohort showed marked overexpression of interferon-stimulated genes (ISGs) and to a lesser extent of inflammation-genes at day 7, which normalized one month post-vaccination. Single-cell RNA sequencing in peripheral blood mononuclear cells from a second cohort identified at baseline a predominantly naive immune landscape including ISGhi cells. On day 7, increased expression of interferon-, inflammation-, and cytotoxicity-related genes were observed in most immune cells, that reverted one month post-vaccination, when a CD8+ ISGhi and cytotoxic cluster and B cells expanded. Antibody responses were associated with baseline frequencies of plasma cells, B-cells, and monocytes, and induction of ISGs at day 7.

Authors: Nouri N, Cao RG, Bunsow E, Nehar-Belaid D, Marches R, Xu Z, Smith B, Heinonen S, Mertz S, Leber A, Smits G, van der Klis F, Mejías A, Banchereau J, Pascual V, Ramilo O,
Journal: Nat Commun;2023Dec02; 14 (1) 7976. doi:10.1038/s41467-023-43758-2
Year: 2023
PubMed: PMID: 38042900 (Go to PubMed)