Late inflammatory monocytes define circulatory immune dysregulation observed in skin microbiome-stratified atopic dermatitis.
Abstract
Atopic dermatitis (AD) is a skin in ammatory disorder well described for signi cant disease heterogeneity. (1) Previously, we de ned steady-state microbial con gurations dermotypes A and B that robustly re ected heterogeneity in AD clinical severity, cutaneous barrier properties and skin microbiome composition. (2) Here, we explored circulating immune dysregulation underlying dermotype-strati ed AD. We performed single-cell RNA sequencing (scRNA-seq) of PBMCs from healthy subjects with dermotype A (n = 4) and AD patients with dermotypes A (n = 4) and B (n = 6). UMAP reduction distinguished 13 major immune clusters using transcriptional pro le di erences, identi ed using DISCO CELLiD (3) and top di erentially expressed genes (DEGs; Supplementary Figure 1A ). Major clusters corroborated well across all groups as a testimony to limited sample distortion by inter-individual or batch e ects (Supplementary Figure 1B ). Critically, AD patients harboured a marked enrichment in monocyte population cluster 1, whereby dermotype strati cation saw a further enrichment in dermotype B
| Authors: | Chua C, Sethi R, Ong J, Low JH, Yew YW, Tay A, Howland SW, Ginhoux F, Chen J, Common JEA, Andiappan AK, |
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| Journal: | J Dermatol Sci;2023Nov03. doi:10.1016/j.jdermsci.2023.10.006 |
| Year: | 2023 |
| PubMed: | PMID: 37989666 (Go to PubMed) |