Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination.
Abstract
Vaccination by inactivated vaccine is an effective strategy to prevent the COVID-19 pandemic. However, the detailed molecular immune response at single-cell level is poorly understood. In this study, we systematically delineated the landscape of the pre- and post-vaccination single-cell transcriptome, TCR (T cell antigen receptor) and BCR (B cell antigen receptor) expression profile of vaccinated candidates. The bulk TCR sequencing analysis of COVID-19 patients was also performed. Enrichment of a clonal CD8+ T cell cluster expressing specific TCR was identified in both vaccination candidates and COVID-19 patients. These clonal CD8+ T cells showed high expression of cytotoxicity, phagosome and antigen presentation related genes. The cell-cell interaction analysis revealed that monocytes and dendritic cells could interact with these cells and initiate phagocytosis via ICAM1-ITGAM and ITGB2 signaling. Together, our study systematically deciphered the detailed immunological response during SARS-CoV-2 vaccination and infection. It may facilitate understanding the immune response and the T-cell therapy against COVID-19.
| Authors: | Cao X, Chen X, Zhu Y, Gou X, Yan K, Yang B, Men D, Liu L, Zhang YA, Cao G, |
|---|---|
| Journal: | Genes Dis;2023 Jul;10(4):1675-1686. doi:10.1016/j.gendis.2022.08.020 |
| Year: | 2023 |
| PubMed: | PMID: 36097543 (Go to PubMed) |