Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock


The immune-suppressive landscape in lepromatous leprosy revealed by single-cell RNA sequencing.


Lepromatous leprosy (L-LEP), caused by the massive proliferation of Mycobacterium leprae primarily in macrophages, is an ideal disease model for investigating the molecular mechanism of intracellular bacteria evading or modulating host immune response. Here, we performed single-cell RNA sequencing of both skin biopsies and peripheral blood mononuclear cells (PBMCs) of L-LEP patients and healthy controls. In L-LEP lesions, we revealed remarkable upregulation of APOE expression that showed a negative correlation with the major histocompatibility complex II gene HLA-DQB2 and MIF, which encodes a pro-inflammatory and anti-microbial cytokine, in the subset of macrophages exhibiting a high expression level of LIPA. The exhaustion of CD8+ T cells featured by the high expression of TIGIT and LAG3 in L-LEP lesions was demonstrated. Moreover, remarkable enhancement of inhibitory immune receptors mediated crosstalk between skin immune cells was observed in L-LEP lesions. For PBMCs, a high expression level of APOE in the HLA-DRhighFBP1high monocyte subset and the expansion of regulatory T cells were found to be associated with L-LEP. These findings revealed the primary suppressive landscape in the L-LEP patients, providing potential targets for the intervention of intracellular bacteria caused persistent infections.

Authors: Mi Z, Wang Z, Xue X, Liu T, Wang C, Sun L, Yu G, Zhang Y, Shi P, Sun Y, Yang Y, Ma S, Wang Z, Yu Y, Liu J, Liu H, Zhang F,
Journal: Cell Discov;2022Jan11; 8 (1) 2. doi:10.1038/s41421-021-00353-3
Year: 2022
PubMed: PMID: 35013182 (Go to PubMed)