Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock


Flow cytometric assessment of leukocyte kinetics for the monitoring of tissue damage.


Leukocyte populations quickly respond to tissue damage, but most leukocyte kinetic studies are not based on multiparameter flow cytometry. We systematically investigated several blood leukocyte populations after controlled tissue damage. 48 patients were assigned to either an anterior or posterolateral total hip arthroplasty. Peripheral blood was collected pre-operatively and at 2 h, 24 h, 48 h, 2 and 6 weeks postoperatively and assessed by flow cytometry for absolute counts of multiple leukocyte populations using standardized EuroFlow protocols. Absolute counts of leukocyte subsets differed significantly between consecutive time points. Neutrophils increased instantly after surgery, while most leukocyte subsets initially decreased, followed by increasing cell counts until 48 h. At two weeks all leukocyte counts were restored to pre-operative counts. Immune cell kinetics upon acute tissue damage exhibit reproducible patterns, which differ between the leukocyte subsets and with "opposite kinetics" among monocyte subsets. Flow cytometric leukocyte monitoring can be used to minimally invasively monitor tissue damage.

Authors: van den Bossche WBL, Rykov K, Teodosio C, Ten Have BLEF, Knobben BAS, Sietsma MS, Josiassen K, de Bruin-Versteeg S, Orfao A, van Dongen JJM, van Raay JJAM
Journal: Clin Immunol. 2018 Dec;197:224-230. doi: 10.1016/j.clim.2018.09.014. Epub 2018
Year: 2018
PubMed: PMID: 30290225 (Go to PubMed)