Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

High-dimensional single-cell analysis reveals the immune characteristics of COVID-19.

Abstract

Coronavirus disease 2019 (COVID-19), driven by SARS-CoV-2, is now declared a global pandemic. Pathogenic T cells and inflammatory monocytes are regarded as the central drivers of the cytokine storm associated with the severity of COVID-19. In this study, we explored the characteristic peripheral cellular profiles of patients with COVID-19 in both acute and convalescent phases by single-cell mass cytometry (CyTOF). Using a combination of algorithm-guided data analyses, we identified peripheral immune cell subsets in COVID-19, and revealed CD4+ T cell depletion, T cell differentiation, plasma cell expansion, and the reduced antigen presentation capacity of innate immunity. Notably, COVID-19 induces a dysregulation in the balance of monocyte populations by the expansion of the monocyte subsets. Collectively, our results represent a high-dimensional single-cell profile of the peripheral immune response to SARS-CoV-2 infection.