Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

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CD14/16 monocyte profiling in juvenile myelomonocytic leukemia.

Abstract

Monocyte subset analysis by flow cytometry has been shown to be a useful diagnostic tool in chronic myelomonocytic leukemia in adults. An increase in the classical monocyte fraction (CD14++/CD16-) greater than 94.0% of total monocytes is considered highly sensitive and specific in distinguishing chronic myelomonocytic leukemia from other myeloproliferative disorders. In a pilot study of juvenile myelomonocytic leukemia cases, we noted that CD14++/CD16- monocyte fraction was >95% in de novo juvenile myelomonocytic leukemia (JMML) with somatic PTPN11 mutations but normal in those with monosomy 7 or Noonan syndrome. Monocyte subgroup profiling by itself is not diagnostic of JMML but may distinguish molecular subgroups within JMML.

Authors: Gadgeel M, Bagla S, Buck S, Shamoun M, Ravindranath Y,
Journal: Pediatr Blood Cancer; 2020 Jul 10 28555. doi:10.1002/pbc.28555
Year: 2020
PubMed: PMID: 32648963 (Go to PubMed)