Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Prognostic value of monocyte subset distribution in chronic myelomonocytic leukemia: results of a multicenter study.

Abstract

To the Editor: According to the World Health Organization (WHO) classification, chronic myelomonocytic leukemia (CMML) diagnosis requires both a persistent peripheral blood monocyte count >1 × 109/L and monocytes accounting for ≥10% of the total white blood cell count (WBC) [1]. We and others have shown that a relative increase in the classical monocyte fraction (cMo; CD14++CD16−) ≥94% of total peripheral blood monocytes, as measured by flow cytometry, rapidly distinguishes a CMML from other causes of monocytosis, mostly reactive monocytosis, with high specificity and sensitivity [2–6].