Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Disappearance of slan-positive non-classical monocytes for diagnosis of chronic myelomonocytic leukemia with an associated inflammatory state

Abstract

The diagnosis of chronic myelomonocytic leukemia(CMML) relies on both a persistent peripheral blood monocytosis (monocytes ≥1x109/L) and monocytes accounting for ≥10% of the white blood cell (WBC) differential count. We showed that a relative accumulation of classical monocytes (cMo: CD14++CD16–) ≥94% among the total peripheral blood monocytes measured by flow cytometry could help to distinguish CMML from other causes of monocytosis. Since then, the quantification of circulating monocyte subsets for the diagnosis of this disease has become widespread. The increase incMo is related to a decrease in the fraction of non-classical monocytes (ncMo: CD14-/lowCD16+).