Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock


Role of stat3 in lipopolysaccharide-induced IL-10 gene expression


IL-10 is a unique cytokine because it is anti-inflammatory and immunosuppressive. IL-10 is regulated at the level of transcription, but the critical motifs and the relevant transcription factors controlling this gene have remained elusive to date. We now report that a sequence at -120 bp in the human IL-10 promoter binds Stat3 but no other Stat proteins. Mutation of this motif abrogates LPS-induced trans-activation. Overexpression of dominant negative Stat3 suppresses promoter activity, while wild-type Stat3 leads to an enhancement of this activity. Our results show that Stat3, by binding to a single motif in the IL-10 promoter, is controlling expression of the human IL-10 gene.  

Authors: Benkhart EM, Siedlar M, Wedel A, Werner T, Ziegler-Heitbrock HW
Journal: J Immunol 165: 1612-1617
Year: 2000
PubMed: Find in PubMed