Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Liposomal 1,25 (OH)₂ vitamin D₃ compounds block proliferation and induce differentiation in myelomonocytic leukaemia cells

Abstract

The vitamin D 3 derived hormone 1,25 (OH)(2) vitamin D 3 (1,25 D 3) is able to induce growth arrest and differentiation in myelomonocytic leukaemic cells. In order to allow for specific delivery to leukaemic cells the lipophilic compound was incorporated into the lipid membranes of liposomes. Liposomal 1,25 D 3 reduced proliferation as measured by H 3 thymidine incorporation in HL60 leukaemia cells by up to 60%. When liposomes were prepared at different concentrations of 1,25 D 3 65% inhibition was achieved at 48 nM. The MC 1288 stereoisomer of 1,25 D 3 was more potent and had the same activity at 4.8 nM.