Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

High CD163+CD206+macrophages infiltration in the bone marrow microenvironment of newly diagnosed multiple myeloma patients.

Abstract

Multiple myeloma (MM) is a neoplastic disease caused by abnormal clonal plasma cells (PCs) in the bone marrow (BM). Macrophages(MFs) are essential components of BM microenvironment and crucial mediators of PCs. Therefore, investigating alterations in the specific MF subsets within the MM BM microenvironment is of significant importance for elucidating the underlying mechanisms of MM. Here, using flow cytometry, we characterized the dynamics of BM MF subsets in newly diagnosed MM (NDMM) patients and complete remission MM (CRMM) patients. We found that NDMM patients exhibited increased infiltration of CD163+CD206+MFs and elevated levels of IL-6 and IL-10 in the BM compared with CRMM patients and healthy controls. BM intermediate monocytes and non-classical monocytes were increased in NDMM patients compared with those in CRMM patients and healthy controls. Furthermore, we observed upregulated C-C motif chemokine receptor 2 (CCR2) and colony-stimulating factor receptor (CSF-R) expression on the BM CD163+CD206+MFs. Notably, the BM CD163+CD206+CCR2+MFs were positively correlated with the increased level of IL-6, while CD163+CD206+CSF-R+MFs were positively correlated with the level of IL-10. Additionally, clinical correlation analysis revealed that an increased proportion of CD163+CD206+CCR2+MFs was negatively correlated with hemoglobin levels and positively correlated with beta2-microglobulin and lactate dehydrogenase. Moreover, a higher proportion of CD163+CD206+CCR2+MFs was detected in the BM of MM patients with poor prognosis. Altogether, our findings suggest that higher proportion of BM CD163+CD206+MFs is involved in pathogenesis of MM, and that CCR2 may be potential therapeutic targets for improving anti-tumor therapy in MM.