Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Lung CD14dimCD16high Nonclassical Monocytes Are Associated with Systemic Inflammation and Cardiovascular Disease Risk in COPD.

Abstract

To the Editor: Cardiovascular diseases (CVDs) are common complications of chronic obstructive pulmonary disease (COPD), which are associated with systemic inflammation, causing the high mortality (1). Nonclassical monocytes (ncMonocytes) maintain vascular homeostasis to protect against the development of CVDs (2). Although ncMonocytes are increased in the peripheral blood of COPD patients (3), their role in systemic inflammation and clinical relevance to CVD risk in COPD remains poorly understood. Here, using flow cytometry (FCM) (4, 5), we characterized lung tissue-derived monocyte subsets to investigate the association of ncMonocytes in systemic inflammation and CVD risk in COPD, and conducted single-cell RNA sequencing (scRNA-seq) to uncover molecular profiles of ncMonocytes. Some results of this study were previously presented in abstract form (6). The experimental design is summarized in Figure E1. Further methodological details are provided in the Supplementary Materials.