Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Single-Cell Sleuthing: Cracking the Monocyte Code for Cardiovascular Clues.

Abstract

Human monocytes were first isolated as Fc receptor– positive and Fc receptor-negative subsets by Zembala and Pryjma1 in 1984. A second seminal discovery in human monocyte biology was the discovery of human clus- ter of differentiation (CD)16+ monocytes in 1989 using 2-color flow cytometry by Passlick and Ziegler-Heitbrock.2 In 2010, the Nomenclature Committee of the International Union of immunologic Societies3 proposed the classical (CD14+), intermediate (CD14+CD16+), and nonclassical (CD16+) nomenclature for human monocytes. In 2003, Geissmann and Jung4 first reported on the mouse equiva- lents of human monocyte subsets. In 2010, Randolph et al5 compared RNA transcriptomes of human and mouse monocyte subsets, demonstrating many similarities in gene expression of monocytes between the 2 species.