Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Assessment of Fcgamma receptor-dependent binding of influenza hemagglutinin vaccine-induced antibodies in a non-human primate model.

Abstract

Several cross-protective antibodies that recognize a broad range of influenza A virus (IAV) strains are known to have functions in virus elimination such as Fcgamma receptor (FcgammaR)-effector function and neutralizing activity against the head region. Although few studies have used primary cells as effector cells, the FcgammaR-effector function was evaluated after isolating each cell subset. Herein, we established an original assay system to evaluate purified FI6 IgG-mediated binding to hemagglutinin (HA)-expressing cells by flow cytometry using peripheral blood mononuclear cells from cynomolgus macaques. In addition, we evaluated the FcgammaR-effector function of IAV vaccine-induced anti-HA antibodies in cynomolgus macaques after administering the split vaccine. We found several cell types, mainly classical monocytes, bound to HA-expressing target cells in an FcgammaR-dependent manner, that were dominant in the binding of the cell population. Thus, this assay system could facilitate the development of a universal influenza vaccine.