Highly efficient antigen targeting to M-DC8+ dendritic cells via Fc-gamma RIII/CD16-specific antibody conjugates
Abstract
Conjugates of peptide antigens with antibodies specifically recognizing surface molecules on dendritic cells (DC) represent an attractive approach to target antigens to antigen-presenting cells (APC) for the induction of specific T cell responses. The present study evaluates the potential of M-DC8(+) DC, a sub-population of professional APC in the blood, for an antibody-based vaccination strategy. We prepared, by chemical cross-linking, conjugates of peptide model antigens with antibodies directed against different cell surface molecules of DC. Antigen-peptide conjugates using an anti-CD16 (FcgammaRIII) antibody were most potent in inducing in vitro activation of a specific CD4(+) T cell response. They were at least 300 times more efficient than two other antibody-antigen conjugates and approximately 500 times more efficient than unconjugated antigen peptides. Our data demonstrate that specific antigen targeting via CD16 on M-DC8(+) DC is a promising vaccination approach for the efficient induction of specific CD4(+) T cell responses ex vivo, and perhaps in vivo.
Authors: | Mende I, Hoffmann P, Wolf A, Lutterbuse R, Kopp E, Baeuerle PA, de Baey A, Kufer P. |
---|---|
Journal: | Int Immunol., 17(5):539-547 |
Year: | 2005 |
PubMed: | Find in PubMed |