Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Oxidized low-density lipoprotein (oxLDL) triggers hypoxia-inducible factor-1a (HIF-1a) accumulation via redox-dependent mechanisms

Abstract

Oxidized low density lipoprotein (oxLDL) and macrophages play a central role in atherosclerosis. Here we obtained evidence that oxLDL induced HIF-1alpha protein accumulation in human macrophages (Mono-Mac-6) under normoxia. HIF-1alpha accumulation was attenuated by pre-treatment with the antioxidant N-acetyl-L-cysteine (NAC), the NO donor S-nitrosoglutathione (GSNO), and NADPH oxidase inhibitors such as diphenyleniodonium (DPI) or 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), thus implicating the contribution of oxLDL-generated reactive oxygen species (ROS). Whereas oxLDL did not modulate HIF-1alpha mRNA levels, experiments with cycloheximide pointed to a translational mechanism in oxLDL action. HIF-1-dependent luciferase reporter gene analysis underscored HIF-1 transactivation. Taken together, our results indicate that oxLDL induced HIF-1alpha accumulation and HIF-1-dependent reporter gene activation in human macrophages via a redox-mediated pathway. This may suggest a role of HIF-1 in atherosclerosis and oxLDL-induced pathogenesis.